Do you expect it to be that quick acting? The study seemed to be what they ate the last 30 days, so I imagined an effect that slowly built up. At the very last some time should be allowed for the bacteria to reach the gut and start colonizing it I think? Also with regards to parties, my experience is that alcohol can affect the gut flora.
The dose can be taken in the morning, given it much of the day to act. Acute effects seem plausible here given the neural connection to the gut, however, a self-experiment examining acute effects only is still useful to help rule that out. And to investigate slower effects, you can block the doses so instead you take a week (or a month) at a time (this is more work because you need more blocks to achieve similar numbers of units as a per-day randomization).
> my experience is that alcohol can affect the gut flora.
So? You don't know if the alcohol effect would be to enhance or reduce the supposed calming effect or not affect that at all.
