In the case of Hacker Dojo, it has big warehouse-style roll-up doors. If the issue were fire safety and egress, an interim policy of only allowing events >49 people when the roller doors are raised seems reasonable.
$250k isn't a huge amount of money, so I don't think there's a huge risk of them getting shut down. Especially given that the space itself is inherently handicap accessible, I don't think ADA bathrooms should be a pressing requirement. Yes, they should have them, but it's not sufficient to deter handicapped people from attending, and it doesn't hurt the value of the space to the majority of non-handicapped attendees.
$250k isn't a huge amount of money, so I don't think there's a huge risk of them getting shut down. Especially given that the space itself is inherently handicap accessible, I don't think ADA bathrooms should be a pressing requirement. Yes, they should have them, but it's not sufficient to deter handicapped people from attending, and it doesn't hurt the value of the space to the majority of non-handicapped attendees.