> Virus propagation in cell culture. Heart tissue from freshly dead Atlantic salmon (Salmo salar L.) was collected from a clinical outbreak of CMS (the diagnosis was confirmed by histopathological examination). The heart tissue was homogenized, followed by centrifugation at 4,000 g and 4°C for 20 min to remove cellular debris, before being processed through a 0.22-m filter. The homogenate was subsequently inoculated onto GF-1 cell cultures (5). The GF-1 cell line, derived from the fin tissue of orange-spotted grouper, Epinephelus coioides, was grown in plug seal cap culture vessels at 15°C after inoculation in Leibowitz 15 supplemented with 1% L-glutamine (2 mM final concentration), 0.1% gentamicin sulfate (50 g/ml final concentration) (all from Sigma Aldrich), and 10% fetal bovine serum (Invitrogen). Development of cytopathic effect (CPE) was monitored at 6, 14, and 21 days postinoculation (p.i.). At 21 days p.i., the supernatant was harvested and passaged further in cell culture.
Notice that since this wasn't a stringent purification they used a subsequent passage to further dilute out anything carrying over. As stated in the other comment it would also be possible to use something such as tangential flow filtration or size-exclusion techniques in order to get an even purer first sample.
https://www.researchgate.net/publication/50409110_Cardiomyop...
> Virus propagation in cell culture. Heart tissue from freshly dead Atlantic salmon (Salmo salar L.) was collected from a clinical outbreak of CMS (the diagnosis was confirmed by histopathological examination). The heart tissue was homogenized, followed by centrifugation at 4,000 g and 4°C for 20 min to remove cellular debris, before being processed through a 0.22-m filter. The homogenate was subsequently inoculated onto GF-1 cell cultures (5). The GF-1 cell line, derived from the fin tissue of orange-spotted grouper, Epinephelus coioides, was grown in plug seal cap culture vessels at 15°C after inoculation in Leibowitz 15 supplemented with 1% L-glutamine (2 mM final concentration), 0.1% gentamicin sulfate (50 g/ml final concentration) (all from Sigma Aldrich), and 10% fetal bovine serum (Invitrogen). Development of cytopathic effect (CPE) was monitored at 6, 14, and 21 days postinoculation (p.i.). At 21 days p.i., the supernatant was harvested and passaged further in cell culture.
Notice that since this wasn't a stringent purification they used a subsequent passage to further dilute out anything carrying over. As stated in the other comment it would also be possible to use something such as tangential flow filtration or size-exclusion techniques in order to get an even purer first sample.