AlphaFold is basically a pattern matching program on steroids. Don't get me wrong, it's incredible, but if there's not experimental data somewhat describing what that variant would look like, AlphaFold is liable to just make spaghetti out of it.
Ugh...ok so not reliable to drive experimental follow ups it seems.
What would it cost to image a certain protein if I sent you the mutant cell lines roughly? I've got 4 mutants whose structure I'm interested in looking at. I can get into more details but essentially, what I'm trying to figure out is if a truncated version of this protein can be created that would expose all the right binding sites and maybe fold somewhat correctly in order to be delivery via AAV for gene therapy.
The mutants I want to look at aren't the truncated protein but the actual diseased types and if I saw structural problems in them, I might assume that fixing said structure could restore function.
Send me an email to the one in my profile - I'd be happy to chat a bit. Not sure a structure is the best way to get the information you're looking for.
